![]() ![]() ![]() To learn more about database construction in general, like integrating contaminant databases or using a decoy strategy for FDR searching, please consult our tutorial on Database Handling. In metaproteomics, choosing the optimal database is a crucial step of your workflow, for further reading see Timmins-Schiffman et al (2017). When using large databases, the peptide identification step can take much more time for computation. Note that sequence databases used for metaproteomics are usually much larger than the excerpt used in this tutorial. The created dataset collection of the three MGF files in the history is used as the MS/MS input. The contaminant database (cRAP) was merged with the original database.įor this, the sequence database-searching program called SearchGUI will be used. The full original database is available online. It is a small excerpt of the original database, which was constructed based on a metagenomic screening of the sea water samples (see May et al. Will be used to match MS/MS to peptide sequences via a sequence database search. The search database labelled FASTA_Bering_Strait_Trimmed_metapeptides_cRAP.FASTA is the input database that You may have to refresh your history to see the queued jobs.Click the Run Workflow button at the top-right of the screen.Click on the workflow-run ( Run workflow) button next to your workflow.You will see a list of all your workflows. Click on Workflow on the top menu bar of Galaxy.
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